Location:Home>Publications

Publication

Publications

Enhanced peroxidase activity and tumour tissue visualization by cobalt-doped magnetoferritin nanoparticles

year:2016Text Size:【big | medium | small】  【print
author:Tongwei Zhang, Changqian Cao, Xu Tang, Yao Cai, Caiyun Yang and Yongxin Pan

        Magnetoferritin (M-HFn) is a biomimetic magnetic nanoparticle with a human heavy-chain ferritin (HFn) shell, trapping a magnetite (Fe3O4) core that has inherited peroxidase-like activity. In this study, cobalt-doped M-HFn nanoparticles (M-HFn-CoxFe3xO4) with different amounts of cobalt were successfully synthesized. Experimental results indicate that the controlled doping of a certain amount of cobalt into the magnetite cores of M-HFn nanoparticles enhances its peroxidase-like catalytic activity and efficacy for visualizing tumour tissues. For example, compared with sample Co0 (without cobalt doping), the peroxidase-like activity of the cobaltdoped nanoparticle sample Co60 (with a cobalt doping molar percentage of 34.2%) increases 1.7 times, and has the maximal reaction velocity (Vmax) values. Moreover, after a one-step incubation with Co60 nanoparticles, and using the peroxidase substrate 3,3-diaminobenzidine tetrahydrochloride (DAB) for colour development, the tumour tissues of breast, colorectal, stomach and pancreas tumours showed a deeper brown colour with clear boundaries between the healthy and tumourous cells. Therefore, this suggests that the cobalt-doped magnetoferritin nanoparticles enhance peroxidase activity and tumour tissue visualization.

 

Download:ZhangTW2016.pdf